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Two adjacent nucleotides in a polynucleotide chain are joined by:
ADisulfide bonds
B3′→5′ phosphodiester linkages
CHydrogen bonds
DPeptide bonds
Answer & Solution
Correct answer: B. 3′→5′ phosphodiester linkages
Nucleotides join via **3′→5′ phosphodiester bonds** — the phosphate at the 5′ carbon of one sugar forms a phosphoester with the 3′-OH of the next sugar. Result: the chain has a free 5′-PO₄ at one end and a free 3′-OH at the other.
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