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The Bohr effect refers to the shift of the O₂-Hb dissociation curve to the RIGHT when

ApO₂ alone increases above 100 mm Hg
BpCO₂ rises, pH falls, or temperature increases
Chaemoglobin concentration falls due to anaemia
Dcarbon monoxide replaces oxygen on heme
Answer & Solution
Correct answer: B. pCO₂ rises, pH falls, or temperature increases
The Bohr effect: rising pCO₂, falling pH (acidosis) or rising temperature reduce Hb's affinity for O₂, shifting the curve right and releasing more O₂ to tissues. Exactly the conditions in an active muscle. CO poisoning is a different mechanism (CO binds with much higher affinity than O₂).
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