Grinding tissue in trichloroacetic acid and filtering separates molecules into
Apolar and non-polar fractions
BDNA and RNA fractions
Cacid-soluble and acid-insoluble pools
Dprimary and secondary metabolites
Answer & Solution
Correct answer: C. acid-soluble and acid-insoluble pools
**The standard syllabus analysis:** grind in trichloroacetic acid, filter.
Filtrate carries small molecules (acid-soluble)
residue on the filter is acid-insoluble macromolecule pool.
Polar vs non-polar is solvent extraction
primary vs secondary is functional, not a separation technique.
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