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Which step of glycolysis is irreversible and a major regulatory point (committed step)?
AGlucose to glucose-6-phosphate
BPGAL to 1,3-BPGA conversion step
CPEP to pyruvate by pyruvate kinase
DFructose-6-P to fructose-1,6-bisphosphate
Answer & Solution
Correct answer: D. Fructose-6-P to fructose-1,6-bisphosphate
Phosphofructokinase-1 (PFK-1) converts F6P to F1,6BP using ATP. It is irreversible and the major rate-limiting step of glycolysis. PFK-1 is allosterically regulated by ATP (inhibitor) and AMP (activator).
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