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In chloroplasts, the Calvin cycle (light-independent reactions) takes place in the
AIntermembrane space
BThylakoid membrane
CStroma
DOuter membrane
Answer & Solution
Correct answer: C. Stroma
The Calvin cycle enzymes (including Rubisco) are located in the stroma. The light reactions occur in the thylakoid membranes, generating ATP and NADPH which the stroma then uses to fix CO2 into carbohydrate.
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