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A fish has a two-chambered heart and single circulation. This means

Ablood passes through the heart once per complete body circuit
Bthe heart pumps only oxygenated blood
Coxygenated and deoxygenated blood never mix
Dblood passes through the heart twice per circuit
Answer & Solution
Correct answer: A. blood passes through the heart once per complete body circuit
In single circulation (fish), blood flows heart → gills → body → heart, passing through the heart once per cycle. Double circulation (mammals) routes blood through the heart twice.
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