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M.Sc Medical Lab Technology Microscopy and Staining — practice questions

Explore 28 free Microscopy and Staining questions in M.Sc Medical Lab Technology Microbiology. Open each question or exercise to read its content, reference answers and available explanation.

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Microscopy and Staining questions and exercises with answers

Showing questions 1–28 of 28.

A technician uses the commonest laboratory microscope, which shows a dark image on a bright background. That instrument is the:Read the answer and worked solution →The light for that instrument comes from a high-intensity bulb sitting below the stage. That part is the:Read the answer and worked solution →The eyepiece lenses of such a microscope typically magnify by:Read the answer and worked solution →The lenses mounted on the rotating nosepiece are the:Read the answer and worked solution →At the highest magnification a drop of oil fills the gap between specimen and lens. That lens is an oil:Read the answer and worked solution →Besides wavelength, one property of a lens governs resolution by measuring its ability to gather light. That property is the numerical:Read the answer and worked solution →A small opaque stop is fitted into the condenser so specimens glow against a dark background. That instrument is the:Read the answer and worked solution →Refraction and interference within a specimen give high contrast without any stain at all in the:Read the answer and worked solution →A microscope that scans several depth planes with a laser to build a three-dimensional picture is the:Read the answer and worked solution →Electron microscopy comes in two basic types: scanning and:Read the answer and worked solution →Before staining, a specimen is attached to the slide by heating or by chemical treatment. That step is:Read the answer and worked solution →The thin layer of specimen spread on the slide before heat fixing is called a:Read the answer and worked solution →A dye absorbed by the cells themselves, making them stand out from the background, is a:Read the answer and worked solution →A dye taken up by the background instead, leaving the organisms as pale outlines, is a:Read the answer and worked solution →Using one dye alone to highlight a structure is called:Read the answer and worked solution →Using several stains so that different organisms can be told apart is called:Read the answer and worked solution →In the Gram procedure, iodine is added after the crystal violet to fix the dye in place. Iodine acts as the:Read the answer and worked solution →Next an alcohol or acetone solution is applied to the smear. That reagent is the:Read the answer and worked solution →Cells with thick peptidoglycan walls resist that step, keeping the crystal violet and appearing:Read the answer and worked solution →Cells with thin peptidoglycan walls lose the first dye and take up the safranin counterstain, appearing:Read the answer and worked solution →Catch the microscope typesRead the answer and worked solution →Pop the Gram reagentsRead the answer and worked solution →Run the laboratory gatesRead the answer and worked solution →Slice the laboratory wordsRead the answer and worked solution →Gram positive or negativeRead the answer and worked solution →Sort the laboratory wordsRead the answer and worked solution →Type the numberRead the answer and worked solution →Microscopy bingoRead the answer and worked solution →

Worked practice questions

A technician uses the commonest laboratory microscope, which shows a dark image on a bright background. That instrument is the:

  1. Brightfield microscope
  2. Blacklight microscope
  3. Bench top spectrometer
  4. Balance beam scale
Show answer and solution

Correct answer: A. Brightfield microscope

1. Two or more lenses are used. 2. The background stays bright. 3. It is the brightfield microscope. _Source: OpenStax Microbiology, Chapter 2, How We See the Invisible World._

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The light for that instrument comes from a high-intensity bulb sitting below the stage. That part is the:

  1. Condenser
  2. Illuminator
  3. Diaphragm
  4. Nosepiece
Show answer and solution

Correct answer: B. Illuminator

1. It supplies the light. 2. It sits under the specimen. 3. It is the illuminator. _Source: OpenStax Microbiology, Chapter 2, How We See the Invisible World._

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The eyepiece lenses of such a microscope typically magnify by:

  1. Ten times
  2. Fifty times
  3. Two times
  4. Five times
Show answer and solution

Correct answer: A. Ten times

1. They work with the objective lenses. 2. The usual figure is standard across labs. 3. It is ten times. _Source: OpenStax Microbiology, Chapter 2, How We See the Invisible World._

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