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Bihar Board Class 12 (Science) Biotechnology and Applications — practice questions — page 4

Explore 129 free Biotechnology and Applications questions in Bihar Board Class 12 (Science) Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 91–120 of 129.

Once activated the Bt toxin kills by acting on the insect:Read the answer and worked solution →Tobacco roots are protected from a nematode by silencing its:Read the answer and worked solution →ELISA works on the principle of interaction between:Read the answer and worked solution →In molecular diagnosis the labelled single strand used is called a:Read the answer and worked solution →Using a country's bio-resources without authorisation is:Read the answer and worked solution →How many documented varieties of Basmati are grown in India?Read the answer and worked solution →The ADA gene is carried into a patient's lymphocytes by a:Read the answer and worked solution →Very low numbers of a pathogen can be detected early by:Read the answer and worked solution →Recombinant DNA uptake is helped by a brief heat shock at:Read the answer and worked solution →The first restriction endonuclease to be isolated was:Read the answer and worked solution →Restriction enzymes are best described as:Read the answer and worked solution →Cutting a stained DNA band out of the gel and recovering it is:Read the answer and worked solution →Human insulin is built from two polypeptide chains joined by:Read the answer and worked solution →The extra stretch removed as pro-insulin matures is the:Read the answer and worked solution →Human insulin was first produced by recombinant means in:Read the answer and worked solution →Inserting a normal gene to correct an inherited defect is:Read the answer and worked solution →The first clinical gene therapy treated a deficiency of:Read the answer and worked solution →Nucleases that remove nucleotides from DNA ends are:Read the answer and worked solution →In gel electrophoresis, DNA fragments move toward the:Read the answer and worked solution →Separated DNA bands become visible after staining with:Read the answer and worked solution →Loss of gene function caused by a DNA insert inside it is:Read the answer and worked solution →Which plasmid was reworked into a cloning vector for plants?Read the answer and worked solution →Restriction enzymes are popularly called:Read the answer and worked solution →Which enzyme is used to open plant cells before DNA isolation?Read the answer and worked solution →Purified DNA finally precipitates out on adding chilled:Read the answer and worked solution →Separation and purification of the final product is called:Read the answer and worked solution →The three sequential steps of one PCR cycle are:Read the answer and worked solution →Taq DNA polymerase used in PCR is obtained from:Read the answer and worked solution →Hind II cuts DNA on recognising a specific sequence of:Read the answer and worked solution →PCR is now used routinely to detect which virus?Read the answer and worked solution →

Worked practice questions

Once activated the Bt toxin kills by acting on the insect:

  1. Midgut cells
  2. Wing muscles
  3. Nerve cords
  4. Egg cases
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Correct answer: A. Midgut cells

1. The active toxin binds the midgut epithelial surface. 2. It creates pores in those cells. 3. The cells swell and burst and the insect dies. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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Tobacco roots are protected from a nematode by silencing its:

  1. Transfer RNA
  2. Chromosomal DNA
  3. Messenger RNA
  4. Ribosomal RNA
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Correct answer: C. Messenger RNA

1. The host is made to produce sense and anti-sense RNA. 2. These pair into a double stranded RNA molecule. 3. That double strand silences the nematode messenger RNA. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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ELISA works on the principle of interaction between:

  1. Antigen and antibody
  2. Enzyme and coenzyme
  3. Hormone and receptor
  4. Solvent and solute
Show answer and solution

Correct answer: A. Antigen and antibody

1. Infection leaves either the pathogen's proteins or the response. 2. The test can pick up either one. 3. It rests on antigen and antibody binding. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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