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Bihar Board Class 12 (Science) Biotechnology and Applications — practice questions — page 2

Explore 129 free Biotechnology and Applications questions in Bihar Board Class 12 (Science) Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 31–60 of 129.

The thermostable polymerase used in PCR is taken from:Read the answer and worked solution →Repeated cycles of PCR can amplify a segment about:Read the answer and worked solution →Which enzyme is used to open plant cells before DNA isolation?Read the answer and worked solution →Purified DNA finally precipitates out on adding chilled:Read the answer and worked solution →Separation and purification of the final product is called:Read the answer and worked solution →The power of any plant cell to grow into a whole plant is:Read the answer and worked solution →Raising thousands of plants through tissue culture is called:Read the answer and worked solution →Plants raised from one parent by tissue culture are called:Read the answer and worked solution →Virus free plants can be raised from an infected plant using its:Read the answer and worked solution →Plant cells stripped of their walls by digestion are called:Read the answer and worked solution →Fusing protoplasts of two varieties gives a plant known as a:Read the answer and worked solution →Rice enriched with vitamin A by genetic modification is:Read the answer and worked solution →The Bt toxin used in pest resistant crops comes from a:Read the answer and worked solution →Inside Bacillus thuringiensis the Bt toxin is stored as:Read the answer and worked solution →The Bt protoxin becomes an active toxin because the insect gut is:Read the answer and worked solution →Once activated the Bt toxin kills by acting on the insect:Read the answer and worked solution →The genes cryIAc and cryIIAb protect cotton against:Read the answer and worked solution →Tobacco roots are protected from a nematode by silencing its:Read the answer and worked solution →Silencing a gene with a complementary double stranded RNA is:Read the answer and worked solution →Human insulin is built from two polypeptide chains joined by:Read the answer and worked solution →The extra stretch removed as pro-insulin matures is the:Read the answer and worked solution →Human insulin was first produced by recombinant means in:Read the answer and worked solution →Inserting a normal gene to correct an inherited defect is:Read the answer and worked solution →The first clinical gene therapy treated a deficiency of:Read the answer and worked solution →The ADA gene is carried into a patient's lymphocytes by a:Read the answer and worked solution →Very low numbers of a pathogen can be detected early by:Read the answer and worked solution →PCR is now used routinely to detect which virus?Read the answer and worked solution →ELISA works on the principle of interaction between:Read the answer and worked solution →In molecular diagnosis the labelled single strand used is called a:Read the answer and worked solution →Most transgenic animals produced so far have been:Read the answer and worked solution →

Worked practice questions

The thermostable polymerase used in PCR is taken from:

  1. Bacillus subtilis
  2. Rhizobium meliloti
  3. Clostridium tetani
  4. Thermus aquaticus
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Correct answer: D. Thermus aquaticus

1. Each cycle heats the DNA until the strands separate. 2. An ordinary polymerase would be destroyed by that heat. 3. Thermus aquaticus supplies one that survives it. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Repeated cycles of PCR can amplify a segment about:

  1. A billion times
  2. A hundred times
  3. A dozen times
  4. A single time
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Correct answer: A. A billion times

1. Every cycle roughly doubles the copies present. 2. Doubling repeated many times grows very fast. 3. The segment ends up amplified about a billion times. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Which enzyme is used to open plant cells before DNA isolation?

  1. Protease
  2. Cellulase
  3. Lysozyme
  4. Chitinase
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Correct answer: B. Cellulase

1. The wall must be digested to release the DNA. 2. Plant walls are built of cellulose. 3. Cellulase is therefore the enzyme used. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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