A limitation of Sanger's original sequencing method is that it can read:

1. Sequencing throughput is measured by how much can be read in parallel. 2. Sanger's method reads a template and reports the order of its bases. 3. However, only one strand at a time can be sequenced at a time. 4. Being able to sequence more than one strand at once would be less time consuming. 5. That constraint is exactly what later high-throughput methods removed. _Source: OpenStax Biology for AP(R) Courses (CC BY 4.0), Ch 14 'DNA Structure and Function', sections 14.1-14.6_