Home › AP Biology › Biology › DNA Structure and Function › A limitation of Sanger's original sequencing met…
A limitation of Sanger's original sequencing method is that it can read:
AOnly very short fragments
BOnly bacterial genomes
COnly the coding strand
DOne strand at a time only
Answer & Solution
Correct answer: D. One strand at a time only
1. Sequencing throughput is measured by how much can be read in parallel.
2. Sanger's method reads a template and reports the order of its bases.
3. However, only one strand at a time can be sequenced at a time.
4. Being able to sequence more than one strand at once would be less time consuming.
5. That constraint is exactly what later high-throughput methods removed.
_Source: OpenStax Biology for AP(R) Courses (CC BY 4.0), Ch 14 'DNA Structure and Function', sections 14.1-14.6_
Related questions
Rosalind Franklin did not share the Nobel Prize for the DNA structure because:The phosphodiester bond of the DNA backbone joins which two carbons?How many base pairs are there in one complete turn of the DNA helix?Each base pair sits how far from the next along the helix?Purines differ from pyrimidines structurally because purines have:The two strands of a DNA double helix are antiparallel, meaning one runs 5' to 3' and the In the Hershey and Chase centrifugation, the heavier bacterial cells ended up in the:The Hershey and Chase experiment could label phage DNA specifically using radioactive phos