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The sixth step of glycolysis adds a second phosphate group to the sugar. What is notable about that phosphate?

AIt comes from the breakdown of a fatty acid
BIt comes from the reduction of an amino group
CIt comes from an inorganic ion, not from ATP
DIt comes from a molecule of carbon dioxide
Answer & Solution
Correct answer: C. It comes from an inorganic ion, not from ATP
1. In step six the sugar is oxidized and its high-energy electrons are picked up by the oxidized carrier, producing NADH. 2. The sugar is then phosphorylated by addition of a second phosphate group, producing 1,3-bisphosphoglycerate. 3. That second phosphate group does not require another adenosine triphosphate molecule. 4. This is why the pathway can turn a profit later, since only two adenosine triphosphate molecules were ever invested. 5. Carbon dioxide is never a phosphate donor, so that option confuses two different atoms. 6. Fatty acid breakdown feeds carbon into a later pathway and supplies no phosphate here. _Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 7 "Cellular Respiration", section 7.2 Glycolysis_
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