Which enzyme cleaves the six-carbon sugar of glycolysis into two three-carbon isomers?
AEnolase
BIsomerase
CHexokinase
DAldolase
Answer & Solution
Correct answer: D. Aldolase
1. The newly added high-energy phosphates destabilise fructose-1,6-bisphosphate.
2. The fourth step employs aldolase to cleave it into two three-carbon isomers.
3. Those isomers are dihydroxyacetone-phosphate and glyceraldehyde-3-phosphate.
4. Enolase acts much later, removing water to make phosphoenolpyruvate.
_Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 7 "Cellular Respiration", section 7.2 Glycolysis_
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